Quantitation of DNA using Hoechst 33258
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چکیده
An essential element of cellular and molecular biology is the ability to quantitate DNA in large numbers of samples at a sensitivity that enables determination of small amounts of sample. Here we describe a fluorescent method to quantitate DNA with Hoechst 33258 dye and the BioTek FL600 fluorescence microplate reader. This assay provides linear results over three orders of magnitude and can routinely detect 8 ng of DNA. Reduction of the dye concentration was found to improve sensitivity to as low as 600 pg/well.
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